2 edition of Observations on the behaviour of avian sternal chondrocytes in three-dimensional collagen gels. found in the catalog.
Observations on the behaviour of avian sternal chondrocytes in three-dimensional collagen gels.
Sheena Francis McClure
Thesis (M.Sc.), - University of Manchester, Faculty of Medicine.
|Contributions||University of Manchester. Faculty of Medicine.|
|The Physical Object|
|Number of Pages||146|
1. Introduction. Tissue engineering (TE) is a form of regenerative medicine that utilizes cells, engineering materials, and suitable biochemical factors to repair, improve, or replace biological tissues. 1 Beyond engineering healthy or developing tissue, some TE approaches seek to create models of tissue disease, dysfunction, or injury to enable mechanistic in vitro exploration. 2 Creating. As the most common chronic degenerative joint disease, osteoarthritis (OA) is the leading cause of pain and physical disability, affecting millions of people worldwide. Mainly characterized by articular cartilage degradation, osteophyte formation, subchondral bone remodeling, and synovial inflammation, OA is a heterogeneous disease that impacts all component tissues of the articular joint.
Sternal plates and ribs are absent in all specimens of Anchiornis and Sapeornis regardless of preservational quality. Sternal morphology is affected by ontogeny, but sternal ossifications appear fairly early in basal birds, present in all known enantiornithine hatchlings and subadult jeholornithiform and ornithuromorph specimens (21, 28 –30). Key words: Human chondrocytes, Immortalization, HPV E6-E7, Collagen II. Introduction Hyaline articular cartilage is a highly specialized tissue derived from mesenchyme during embryonic develop-ment1. Chondrocytes, the only cell type of mature cartilage, secrete and deposit around themselves a .
Objective. To investigate in vivo simulation of the microenvironment in which osteoarthritis (OA) chondrocytes are cultured in vitro. Methods. Human articular chondrocytes were cultured under normoxic and hypoxic conditions. Cells were cultured on standard culture plastic or a porous polyHEMA surface that closely resembles the in vivo cartilage microarchitecture. Three-dimensional imaging by helical CT was used in the evaluation of anterior chest wall deformities during childhood without focusing on the sternum by 3D or multiplanar imaging. Three-dimensional CT images of the sternum were obtained in a few cases with sternal anomalies, such as complete sternal .
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Brian K. Hall, in Bones and Cartilage, Collagen gel culture. Similarly, sternal chondrocytes from day-old embryos retain their differentiated state and continue to deposit ECM when cultured in collagen can synthesize additional collagen types if cultured in collagen gels, fibronectin modifying the type of collagen synthesized, two disulphide-linked molecules being produced.
Interestingly, when the immature caudal sternal chondrocytes were mixed and cocultured with cephalic chondrocytes in agarose they suppressed the IGF‐1–stimulated maturation process of the cephalic cells, as indicated by inhibition of type X collagen synthesis and alkaline phosphatase activity.
10 Furthermore, the immature cells were found Cited by: Chondrocytes isolated from sternal cartilage of day embryonic chicks were dispersively embedded inside the collagen gels in Ham's F medium containing fetal calf serum. Embryonic chick sternal chondrocytes were cultured either within three dimensional gels of type I collagen, type II collagen or agar, or as monolayers on plastic dishes coated with air-dried films of these matrix macromolecules.
It was observed that cell shape and cell growth varied markedly between the different culture conditions. Chondrocytes from the cephalic region of day chick embryo sterna were cultured within type I collagen gels in the presence of increasing concentrations of calcium beta-glycerophosphate (Ca beta.
Bovine chondrocytes seeded at low density ( x 10(4) cells/ml of gels) in three-dimensional collagen type I gels do proliferate and maintain their phenotype as shown by cell counts, morphology. Chick-embryo sternal chondrocytes have been cultured within three-dimensional collagen gels as part of a study concerned with the effects of extracellular matrix macromolecules on chondrocyte gene.
Transforming growth factor β (TGF‐β) has well‐documented roles in chondrocyte maturation and endochondral ossification, but the mechanisms of TGF‐β activation during these. McClure J, Bates GP, Rowston H, Grant M: A comparison of the morphological, histochemical and biochemical features of embryonic chick sternal chondrocytes in vivo with chondrocytes cultured in three-dimensional collagen gels.
Bone Mineral 3: –, Google Scholar. Cl--HCO3- exchanger is the main chondrocytes acid loader. Cells were maintained in a HCsolution and the pH. was varied by acid addition (pH0valuesofand). Afteraninitial acidification, thepHireturned within min to its initial value of about We conclude that in avian chondrocytes a se is opposed byeffective acid.
In this contex, Hyalograft ® C Autograft, a commercial three-dimensional HYAFF11 scaffolds enriched with autologous chondrocytes, has reveled in clinical medium-term results on the treatment of 0.
The major band in avian embryonic cartilage has a molecular weight of approximat No mutations affecting link protein have been described. Collagen. The predominant type of collagen synthesized by chondrocytes is genetically distinct from that synthesized by other tissues (Miller, ; and see Volume 1, Chapter.
Schuman L, Buma P, Versleyen D. Chondrocyte behaviour within different types of collagen gels in vitro. Biomaterials. ; – doi: /(95) Schmidt-Rohlfing B, Silny J, Woodruff S, Gavenis K.
Effects of pulsed and sinusoid electromagnetic fields on human chondrocytes cultivated in a collagen matrix. Objective: Using the alginate bead three-dimensional culturing method, which is considered to be advantageous for the in vitro study of chondrocytes, we confirmed earlier reports concerning the inhibitory effect of TGF-β on IL-1β-induced cartilage destruction and serially evaluated changes in proteinases and their inhibitors in cartilage destruction.
The density of cells in a given tissue volume depended on the growth stage and the depth from the articular surface (Fig. 1, Fig. 2, Fig. 3).The cellularity of articular cartilage decreased markedly with maturation (p.
Tendon tissue engineering based on a commercially available collagen type I-gel has been reported to lead to times higher ultimate stress and to times higher elastic modulus (Table a) when avian tendon internal Fbs were seeded and uniaxially loaded for 1 h/day and 1% elongation at 1 Hz in a bioreactor (Garvin et al., ).
Isolation and Propagation of Articular Chondrocytes. Adult articular chondrocytes were isolated as previously described ().Briefly, cartilage biopsies were collected from distal femurs and cut into 1–mm 3 pieces. Enzymatic digestion of tissues was carried out for 18 h in 2–5 ml DMEM/Ham's F (Cat.
TBioChrom Labs, Terre Haute, IN, USA) containing collagenase as. Development of three-dimensional scaffolds mimicking in vivo cells’ environment is an ongoing challenge for tissue engineering.
Bacterial nano-cellulose (BNC) is a well-known biocompatible material with enormous water-holding capacity. However, a tight spatial organization of cellulose fibers limits cell ingrowth and restricts practical use of BNC-based scaffolds.
β-Catenin regulates important biological processes, including embryonic development and tumorigenesis. We have investigated the role of β -catenin in the regulation of the chondrocyte phenotype. Expression of β -catenin was high in prechondrogenic mesenchymal cells, but significantly decreased in differentiated chondrocytes both in vivo and in vitro.
Nanocellulose is cellulose in the form of nanostructures, i.e., features not exceeding nm at least in one dimension. These nanostructures include nanofibrils, found in bacterial cellulose; nanofibers, present particularly in electrospun matrices; and nanowhiskers, nanocrystals, nanorods, and nanoballs.
These structures can be further assembled into bigger two-dimensional (2D) and three. SOX-9 was immunolocalised to majority of the chondrocytes of the pellets, and prominent presence of collagen Type II was observed in the chondrocytes as well as the surrounding extracellular matrix.
Furthermore, the pellets demonstrated negligible staining for collagen Type I, a constituent of fibrocartilage, and collagen Type X, a phenotypic.
Microscopic observation of chondrocytes at the gate. (a) Without APM and ITS. (b) With APM and ITS. In (a) and (b), the morphological properties of chondrocytes at the gate were observed at 72,and h.
W and L in (a) denote the width and length of the gate used in this study.This review focuses on the expanding role of marine collagen (MC)-based scaffolds for biomedical applications. A scaffold—a three-dimensional (3D) structure fabricated from biomaterials—is a key supporting element for cell attachment, growth, and maintenance in 3D cell culture and tissue engineering.
The mechanical and biological properties of the scaffolds influence cell morphology.